非酶反应体系过氧化氢含量荧光测定方法研究及分析应用

本文基于Fe（Ⅱ）与H2O2在酸性介质中经Femon反应产生羟基自由基,与苯甲酸发生羟基化反应产生荧光产物,建立了一种测定过氧化氢的荧光分析方法。该方法具有简便、快速、灵敏度高的特点,试用于雨水中过氧化氢含量的测定,获得了满意的结果。     

卤虫携带白斑综合征病毒的研究

卤虫是是,蟹苗种培育的关键动物性饵料,查清其能否携带白斑综合征病毒,对培育健康苗种至关重要。2002年7月至10月,自盐田中取野生卤虫,经暂养后选20尾抱卵雌虫,每尾置1个烧杯中,投喂单胞藻,产卵后取出亲体;20d后,每个烧杯中取5尾子一代个体,利用巢式体外DNA扩增技术对亲体和子一代成体进行WSSV病毒检测,检测结果显示：卤虫中可扩增出特异性的,与引物设计吻合的DNA片段;DNA测序分析表明,扩增片段的DNA序列与白斑综合征病毒序列一致;野生卤虫携带白斑综合征病毒的阳性率为58%,实验条件下野生卤虫后代的阳性率为43%,同一家系中,亲代与子代携带病毒的情况不完全一致,不能确实白斑综合征病毒可否通过繁殖,在卤虫世代间进行垂直传播,研究结果表明：卤虫很可能是白斑综合征病毒的携带者,苗种培育和养殖期间投喂卤虫可能造成虾,蟹感染白斑综合征病毒,卤虫检疫是培育健康苗种的关键措施。     

湖北发生的水稻矮缩病是南方水稻黑条矮缩病毒引起的

 近年来由水稻黑条矮缩病毒和南方水稻黑条矮缩病毒引起的水稻黑条矮缩病在华南、华东等地区暴发流行,给水稻生产带来严重损失,而湖北一直未有水稻黑条矮缩病流行的报道。然而,2009年和2010年在湖北省长江沿线的荆州、孝感、咸宁、黄石、鄂州、黄冈等水稻种植区普遍发生水稻矮缩减产的现象。经过症状观察、dsRNA电泳图谱分析、RT-PCR等方法,诊断该病是由南方水稻黑条矮缩病毒引起,说明该病毒已经随介体昆虫白背飞虱从我国南方传播至中部水稻种植区。     

Degradation of chlorophenoxyacid herbicides in aqueous media,using a novel electrochemical method†

The degradation of five chlorophenoxyacid herbicides has been studied using an electrochemical method based on the Fenton reaction (simultaneous reduction of dioxygen and ferric ions). The method consists of electrosynthesizing OH^· radicals, which react rapidly with chlorophenoxyacids in aqueous media. HPLC and GC-MS analysis show the formation of polyhydroxyphenols and quinones in a first step, and the complete destruction of the aromatic nucleus upon exhaustive electrolysis. This offers a possible way for the depollution of natural waters containing chlorophenoxyacid pesticide residues. © 1999 Society of Chemical Industry.     

The isolated,perfused bovine ear. A model for pharmacological study of cutaneous vasculature and anaphylaxis

Bovine ears were catheterized and perfused with Krebs-Henseleit (K-H) solution at a constant flow rate. The perfusion pressure (PP) was monitored using a pressure transducer coupled to a carrier preamplifier, amplifier and a pen recorder. Dose-response curves to phenylephrine, histamine and 5-hydroxytryptamine were obtained. The ear preparations responded to these agonists dose-dependently by increases in perfusion pressure. Ear preparations from sensitized calves were challenged with 0.05 ml of horse serum which caused increased perfusion pressure. These preliminary results suggest that the perfused bovine ear represents a promising, simple, inexpensive model for the study of pharmacological mechanisms of the skin.     

ω-酰基莰烯系列化合物的合成、香气与分析

通过ω-甲酰基莰烯与格氏试剂反应,然后水解和氧化,合成了13种ω-酰基莰烯,并进行了MS、IR、~1H NMR分析和评香鉴定。     

Repeated sequence RSα is diagnostic for Bradyhizobium japonicum and Bradyrhizobium elkanii

The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Bradyrhizobium strains capable of nodulating soybean was also demonstrated. Received: 11 May 1995     

离子束修饰析氢电极的研究

The article concentrated on the modifying effects of ion beam mixing, ion implanting and ion sputtering of hydrogen evolution electrodes. It was discovered that the four types of electrodes possessed excellent catalytic activity in both acid and alkaline